- Interim Director/Adjunct Research Professor in Chemistry
- Environmental Toxicology and Chemistry Laboratory
- Great Lakes Center
SUNY College at Buffalo - Science Building 267
- 1300 Elmwood Avenue
- Buffalo, New York 14222-1095
- Telephone: 716-878-5422
- Fax: 716-878-5400
- E-mail: kumars@buffalostate.edu
EDUCATION:
1974-1977: Post-doctoral training; Organic synthesis, Ohio State University, Columbus, Ohio
1971: Ph.D.; Chemistry University of Lucknow, Lucknow, India
1967: M.Sc.; Organic Chemistry University of Lucknow, Lucknow, India
1964: B.Sc.; Chemistry, Physics and Maths, University of Lucknow, Lucknow, India
RESEARCH INTERESTS:
Mechanism of action of chemical carcinogens; development of novel synthetic methods of compounds of interest in cancer research; synthesis and structural identification of the stereoisomeric metabolites and DNA adducts of environmental carcinogens and toxicants; chemical and toxicological studies of polybrominated diphenyl ethers in fish and mammalian species; development of mechanism-based anti-cancer and chemopreventive agents; characterization of organic contaminants in various environmental samples
HONORS, AWARDS AND OTHER PROFESSIONAL ACTIVITIES:
Principal and co-investigator of research grants awarded by National Institutes of Health (NIH), U. S. Environmental Protection Agency, and other federal and private agencies since 1983.
Research Foundation at State University College at Buffalo award for “Outstanding Contributions to Buffalo State College and Efforts, Persistence and Success in Sponsored Program Research”, April 2002
SUNY Chancellor’s Research Recognition Award for “exemplary contribution to research at the State University of New York”, October 2002.
“The Million Dollar Club” member of the Research Foundation, Buffalo State College, April, 2006
Expert reviewer for specific grant applications from U.-S. Israel Binational Science Foundation, January, 1990; Chemical Pathology Study Section (CPA)/NIH, January, 1991; Epidemiology and Disease Control Study Section/NIH, October, 1996.
Reviewer for cancer research program project, NCI/NIH, February, 1997, February, 2001, February, 2002; for exposure assessment methods for cancer research, NCI/NIH, July, 2001; for cancer prevention research small grant program/small grant program for cancer epidemiology, NCI/NIH, February, 2002.
One hundred and six publications in peer-reviewed journals, and 69 presentations in National and International Scientific conferences.
GRANTS/CONTRACTS RECEIVED (Last 5 years):
2001-2005; Mutagenesis of Thia-PAHs with Peripheral Thiophene Ring. Philip Morris External Research Program.
2002-2005: Disposition and Metabolism of Polybrominated Diphenyl Ethers in Fish. New York Sea Grant Institute.
2002-2005: Effect of Postnatal Development on the Metabolism of PAHs and the Repair of PAH-induced DNA damage. Philip Morris External Research Program.
2001-2003: Mechanism of Synergistic Interaction of Tobacco Smoke Constituents. Philip Morris External Research Program.
SELECTED REVIEWED PUBLICATIONS:
S. Kumar and S. Saravanan. A facile synthesis of 11,12-dimethoxydibenzo[def,p]-chrysene, a preferred precursor for the synthesis of the proximate and ultimate carcinogens of dibenzo[def,p]chrysene (DBC). Polycyclic Aromatic Compounds, in press (2009).
J. J. Mukherjee, S. K. Gupta, and S. Kumar. Inhibition of benzopyrene-diol epoxide (BPDE)-induced bax and caspase-9 by cadmium: role of mitogen activated protein kinase. Mutation Res. 661, 41-46 (2009).
C. D. Swartz, L. C. King, S. Nesnow, D. M. Umbach, S. Kumar, and D. M. DeMarini. Mutagenicity, stable DNA adducts, and abasic sites induced in Salmonella by phenanthro[3,4-b]- and phenanthro[4,3-b]thiophenes, sulfur analogs of benzo[c]phenanthrene. Mutation Res. 661, 47-56 (2009).
Y. Wang, N. C. Schnetz-Boutaud, H. Kroth, Y. Yagi, J. M. Sayer, S. Kumar, D. M. Jerina, and M. P. Stone. 3’-Intercalation of a N2-dG 1R-trans-anti-benzo[c]phenanthrene DNA adduct in an iterated (CG)3 repeat. Chem. Res. Toxicol. 21, 1348-1358 (2008).
J. J. Mukherjee, S. K. Gupta and S. Kumar. Inhibition of benzopyrene diol epoxide-induced apoptosis by cadmium (II) is AP-1-independent: role of extracellular signal related kinase. Chemico-Biol. Interact. 172, 72-80 (2007).
K. K. Laali, J.-H. Chun, T. Okazaki, S. Kumar, G. L. Borosky, and C. Swartz. Electrophilic chemistry of thia-PAHs: Stable carbocations (NMR and DFT), S-alkylated onium salts, model electrophilic substitutions (nitration and bromination) and mutagenicity assay. J. Org. Chem. 72, 8383-8393 (2007).
V. K. Batra, D. D. Shock, R. Prasad, W. A. Beard, E. W. Hou, L. C. Pedersen, J. M. Sayer, H. Yagi, S. Kumar, D. M. Jerina, and S. H. Wilson. Structure of DNA polymerase β with a benzo[c]phenanthrene diol epoxide adducted template exhibits features that can lead to mutagenesis. Proc. Natl. Acad. Sci. 103, 17231-17236 (2006).
S. Kumar, S. Saravanan, P. Reuben, and A. Kumar. Synthesis of trans-dihydrodiol derivatives of phenanthro[3,4-b]thiophene and phenanthro[4,3-b]thiophene. J. Heterocycl. Chem. 42, 1345 (2005).
S. Kumar, A. Kumar, and H. C. Sikka. Comparative metabolism of phenanthro[3,4-b]thiophene, phenanthro[4,3-b]thiophene and their carbon analog benzo[c]phenanthrene by rat liver microsomes. Polyclic Arom. Comp. 24, 527 (2004).
S. Kumar, P. Reuben, and A. Kumar. Synthesis of dihydrodiols of phenanthro[3,4-b]thiophene and phenanthro[4,3-b]thiophene. Polyclic Arom. Comp. 24, 289 (2004)
Z.-X. Yuan, S. Kumar and H. C. Sikka. Comparative metabolism of the aza-polynuclear aromatic hydrocarbon dibenz[a,h]acridine by recombinant human and rat cytochrome P450s. Chem.Res.Toxicol. 17, 672 (2004).
J. J. Mukherjee, S. K. Gupta, S. Kumar, and H. C. Sikka. Effects of cadmium (II) on (±)-anti-benzo[a]pyrene-7,8-diol-8,9-epoxide-induced DNA damage response in human fibroblasts and DNA repair: A possible mechanism of cadmium’s cogenotoxicity. Chem. Res. Toxicol. 17, 287 (2004).
A. K. Sharma, S. Kumar and S. Amin. A highly abbreviated synthesis of dibenzo[def,p]chrysene. J. Org. Chem. 69, 3979 (2004).
S. Kumar, J.-M. Lin, J. Whysner, H. C. Sikka and S. Amin. Mutagenicity of benzo[b]phenanthro[2,3-d]thiophene (BPT) and its metabolites in TA100 and base-specific tester strains (TA7001-TA7006) of Salmonella typhimurium: Evidence of multiple pathways for the bioactivation of BPT. Mutation. Res. 545, 11 (2004).
Z.-X. Yuan, H. C. Sikka, S. Munir, A. Kumar, A. V. Muruganandam, and S. Kumar. Metabolism of polynuclear sulfur heterocycle benzo[b]phenanthro[2,3-d]thiophene by rodent liver microsomes. Evidence for multiple pathways in the bioactivation of benzo[b]phenanthro[2,3-d]thiophene. Chem.Res.Toxicol. 16, 1581 (2003).
J. Pangrekar, P. L. Kole, S. A Honey, S. Kumar, and H. C. Sikka. Metabolism of phenanthrene by brown bullhead liver microsomes. Aquat. Toxicol. 64, 407 (2003).
N. W. Shappell, U. Carlino-MacDonald, S. amin, S. Kumar, H. C. Sikka. Comparative metabolism of chrysene and 5-methylchrysene by rat and rainbow trout liver microsomes. Toxicol. Sci. 72, 260 (2003).
J. Pangrekar, P. L. Kole, S. A. Honey, S. Kumar, and H. C. Sikka. Metabolism of chrysene by brown bullhead liver microsomes. Toxicol. Sci. 71, 67 (2003).
S. Kumar. Synthesis of dihydrodiol metabolites implicated in the mechanism of carcinogenesis of phenanthro[4,3-b][1]benzothiophene and phenanthro[3,4-b][1]benzothiophene, the polycyclic sulfur heterocycles with a ‘fjord’ structure. J. Org. Chem., 67, 8842 (2002)
H. Yagi, A. R. Ramesha, G. Kalena, S. Kumar, and D. M. Jerina. Novel stereoselective control over cis- versus trans-opening of benzo[c]phenanthrene 3,4-diol 1,2-epoxide by the exocyclic N2-amino group of deoxyguanosine in the presence of hexafluoropropan-2-ol. J. Org. Chem., 67, 6678 (2002).
L. A. Ramos, I. Ponten, A. Dipple, S. Kumar, H. Yagi, J. M. Sayer, H. Kroth, G. Kalena, and D. M. Jerina. Site-specific mutagenesis in E. Coli by N2-deoxyguonosine adducts derived from the highly carcinogenic fjord-region benzo[c]phenanthrene 3,4-diol 1,2-epoxides. Chem. Res. Toxicol., 15, 1619 (2002).
A. K. Sharma, S. Kumar, and S. Amin. An abbreviated synthesis of 7,12-dimethylbenz[a]-anthracene and benzo[c]chrysene metabolites using Suzuki reaction. Polycyclic Aromatic Compounds 22, 277 (2002).
M. A. Siddiqui, Z.-X. Zin, S. Kumar, and H. C. Sikka. Metabolism of PAHs and methyl-substituted PAHs by sphingomonas paucimobilis strain EPA 505. Polycyclic Aromatic Compounds 22, 621 (2002).
S. Kumar, R. l. Chang, A. W. Wood, J. G. Xie, M. T. Huang, X. X. Cui, P. L. Kole, H. C. Sikka, S. K. Balani, A. H. Conney, and D. M. Jerina.Tumorigenicity of racemic and optically pure bay-region diol epoxides and other derivatives of the nitrogen heterocycle dibenz[a,h]acridine on mouse skin. Carcinogenesis 22, 951 (2001).
S. Kumar. Synthesis of trans-3,4-dihydroxy-3,4-dihydrophenanthro[3,2-b][1]benzothiophene, a potentially carcinogenic metabolite of sulfur heterocycle phenanthro[3,2-b][1]benzothiophene. J. Chem. Soc. Perkin Trans. 1018 (2001).
S. Kumar. A convenient and general synthesis of cata- and peri-condensed polycyclic aromatic hydrocarbons with a fjord-region. Synthesis 841 (2001).
H. Kroth, H. Yagi, J. M. Sayer, S. Kumar, and D. M. Jerina. O6-Allyl protected deoxyguanosine adducts of polycyclic aromatic hydrocarbons as building blocks for the synthesis of oligonucleotides. Chem. Res. Toxicol. 14, 708 (2001).
K. C. Fertuck, S. Kumar, H. C. Sikka, J. B. Matthews, and T. R. Zacharewski. Interaction of PAH-related compounds with the α and β isoforms of the estrogen receptor. Toxicol. Lett. 121, 167 (2001).
S. Kumar and T.-Y. Kim. An improved and regiospecific synthesis of trans-3,4-dihydrodiol metabolite of benzo[b]naphtho[2,1-d]thiophene. J. Org. Chem. 65, 3883 (2000).
I. Ponten, J. M. Sayer, A. S. Pilcher, H. Yagi, S. Kumar, D. M. Jerina, and A. Dipple. Factors determining mutagenic potential for individual cis and trans opened benzo[c]phenanthrene diol epoxide-deoxyadenosine adducts. Biochemistry. 39, 4136 (2000).
S. Honey, P. O'keefe, A. T. Drahushuk, J. R. Olson, S. Kumar, and H. C. Sikka. Metabolism of benzo[a]pyrene by duck liver microsomes.Comp. Biochem. Physiol. Part C 126, 285 (2000).
J. D. Adams, A. Chadha, R. E. Lehr, S. Kumar, W. Levin, and D.M. Jerina. The proximate carcinogen, trans-3,4-dihydroxy-3,4-dihydrodibenz[c,h]acridine is oxidized stereoselectively and regioselectively by cytochrome 1A1, epoxide hydrolase and hepatic microsomes from 3-methylcholanthrene pretreated rats. Chem.-Biol. Interac. 122, 117 (1999).
J. M. Arif, N. Shappell, H. C. Sikka, S. Kumar, and R. C. Gupta. 32P-postlabeling analysis of lipophilic DNA adducts resulting from interaction with (±)-3-hydroxy-trans-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene. Chem.-Biol. Interact. 118, 87 (1999).
I. Ponten, J. M. Sayer, A. S. Pilcher, H. Yagi, S. Kumar, D. M. Jerina, and A. Dipple. Sequence context effects on mutational properties of cis-opened benzo[c]phenanthrene diol epoxide-deoxyadenosine adducts in site-specific mutation studies. Biochemistry 38, 1144 (1999).
Z.-X. Yuan, S.A. Honey, S. Kumar, and H.C. Sikka. Comparative metabolism of dibenz[a,l]pyrene by liver microsomes from rainbow trout and rats. Aquat. Toxicol. 45, 1 (1999).
S. Kumar. A new efficient route to the phenolic derivatives of chrysene and 5-methylchrysene, precursors to dihydrodiol and diol epoxide metabolites of chrysene and 5-methylchrysene, through Suzuki cross coupling reaction. J. Chem. Soc. Perkin Trans. I, 3157 (1998).
S. Kumar. A new and concise synthesis of 3-hydroxybenzo[c]phenanthrene and 12-hydroxybenzo[g]chrysene, useful intermediates for the synthesis of fjord-region diol epoxides of benzo[c]phenanthrene and benzo[g]chrysene. J. Org. Chem. 62, 8535 (1997).
S. Kumar. A new abbreviated synthesis of 5-methylchrysene and its 2-hydroxy- and 8-hydroxy derivatives., Tetrahedron Lett. 37, 6271 (1996).
S. Kumar. Studies toward the synthesis of 3,7β,8α-trihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene, a reactive metabolite of benzo[a]pyrene., Tetrahedron Lett. 37, 1527 (1996).
S. Kumar and S. K. Singh. A rapid, convergent and regioselective synthesis of 3,7,8-trihydroxy-trans-7,8-dihydrobenzo[a]pyrene, Tetrahedron Lett. 36, 1213 (1995).
S. Kumar, S. K. Singh, P. L. Kole, S. Elmarakby and H. C. Sikka. Stereoselective metabolism of dibenz[a,h]acridine to its bay-region 10,11-diol-8,9-epoxides by rat liver microsomes, Carcinogenesis (Lond.) 16, 525 (1995).
H. C. Sikka, A. R. Steward, S. A. Elmarakaby, K. G. Stuart, S. Kumar, U. Devanaboyina, and R. C. Gupta Metabolism of 2-acetylaminofluorene by rainbow trout, Marine Environ. Res. 39, 45 (1995).
B. G. Loganathan, K. Kannan, I. Watanabe, M. Kawano, K. Irvine, S. Kumar and H. C. Sikka. Isomer-specific determination and toxic evaluation of polychlorinated biphenyls, polychlorinated/brominated dibenzo-p-dioxins and dibenzofurans, polybrominated ethers, and extractable organic halogen in carp from the buffalo river, New York. Env. Sci. Technol., 29, 1832 (1995).
J. R. Olson, B. P. McGarrigle, P. J. Gigliotti, S. Kumar, and J. H. McReynolds. Hepatic uptake and metabolism of 2,3,7,8-tetrachlorodibenzo-p-dioxin and 2,3,7,8-tetrachlorodibenzofuran, Fundam. Appl. Toxicol. 14, 631 (1994).
R. L. Chang, S. Battista, C. Q. Wong, S. Kumar, P. L. Kole, H. C. Sikka, S. K. Balani, D. M. Jerina, A. H. Conney, and A. W. Wood. Bacterial and mammalian cell mutagenicity of four optically active bay-region 10,11-diol 8,9-epoxides of nitrogen heterocycles dibenz[a,h]acridine, Carcinogenesis 14, 2233 (1993).
S. K. Singh, and S. Kumar. Synthesis of oxygenated derivatives of 2,3,7,8-tetrachlorodibenzo-p-dioxin, J. Agri. Food. Chem., 41, 1511 (1993).
S. Kumar, P. L. Kole, S. K. Balani, and D. M. Jerina. Synthesis of enantiomerically pure bay-region 10,11-diol 8,9-epoxide diastereomers of the carcinogen dibenz[a,h]acridine, J. Org. Chem. 57, 2784 (1992).
P. L. Kole and S. Kumar. Synthesis of dibenz[a,c]anthracene 1,2-oxide and 3,4-oxide from dibromotrifluoro-acetates: concomitant formation of oxepines. J. Chem. Soc. Perkin Trans I, 2151 (1991).
H. C. Sikka, A. R. Steward, C. Kandaswami, J. P. Rutkowski, J. Jalewski, S. Kumar, K. Earley, and R. C. Gupta. Metabolism of benzo[a]pyrene and persistence of DNA adducts in brown bullheads (Ictalurus nebulosus). Comp. Physiol. Biochem. C. 100C. 25 (1991).
H. C. Sikka, C. Kandaswami, J. P. Rutkowski, S. Kumar, K. Earley, and R. C. Gupta. Formation and persistence of DNA adducts in the liver of brown bullheads exposed to benzo[a]pyrene. Cancer Lett. 40, 81 (1990).
P. L. Kole, and S. Kumar. Synthesis of the phenol esters of 1-hydroxy- and 3-hydroxy-7,8-diol-9,10-epoxides of benzo[a]pyrene. Tetrahedron Lett. 3707 (1990).
J. M. Sayer, R. E. Lehr, S. Kumar, H. Yagi, H. J. C. Yeh, G. M. Holder, J. V. Silvertone, C. Gibson, and D. M. Jerina. Effect of aza substitution on the solvolytic activity of bay-region diol epoxides: dibenz[c,h]- and dibenz[a,j]anthracene-3,4-diol-1,2-epoxide. J. Am. Chem. Soc. 112, 1177 (1990).
S. Kumar, P. L. Kole, and H. C. Sikka. Mutagenicity of dibenz[a,c]anthracene and its derivatives in Salmonella Typhimurium TA100. Mutation Res. 242, 337 (1990).
P. L. Kole, S. K. Dubey and S. Kumar. Synthesis of isomeric cis-dihydrodiols and phenols of highly mutagenic dibenz[a,c]anthracene. J. Org. Chem., 54, 845 (1989).
R. K. Sehgal and S. Kumar. A simple preparation of 1-hydroxypyrene. Org. Prep. Proced. Inter., 21, 224 (1989).
S. Kumar, P. L. Kole, and R. K. Sehgal. Synthesis of phenolic derivatives of highly tumorigenic trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene. J. Org. Chem., 54, 5272 (1989).
S. Kumar, H. C. Sikka, S. K. Dubey, A. Czech, N. Geddie, M. Wang, and E. J. LaVoie. Mutagenicity and Tumorigenicity of dihydrodiols, diol epoxides and tetrahydroepoxides of benzo[f]quinoline and benzo[h]quinoline. Cancer Res., 49, 20 (1989).
A. W Wood, R. L. Chang, M. Katz, A. H. Conney, D. M. Jerina, H. C. Sikka, W. Levin and S. Kumar. Mutagenicity of dihydrodiols and diol epoxides of dibenz[a,h]acridine in bacterial and mammalian cells. Cancer Res., 49, 6981 (1989).
C. Kandaswami, J. P. Rutkowski, S. Kumar and H. C. Sikka. Comparative metabolism of benzo[f]quinoline by liver microsomes from rats and brown bullhead. Comp. Biochem. Physiol., 93C, 269 (1989).
PATENTS:
"Synthesis of 8-aza-9-oxo-15-dihydroxyprostanoic acids," S. Kumar, C.M. Gupta, N. Anand, Indian, 142, 125: Oct. 8, 1977; Appl. 26 Sept. 1974, C.A. 93, 7734w (1980).
